Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Frequency of Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and in healthy subjects. ( A ) Distribution of percentages of total lymphocytes, T (CD3+), Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients, compared to healthy subjects. Shown is mean ± SD. ( B ) Representative dot-plots showing the frequency of Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and healthy subjects. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques:

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Phenotypic analysis of circulating Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and in healthy subjects. Distribution of ex vivo memory subsets of Vδ1+ ( A ) and Vδ2+ ( C ) T lymphocytes based on the expression of CD27 and CD45RA in hospitalized and recovered COVID-19 patients, compared to healthy donors. Median is shown. Healthy donors were represented as triangle, COVID-19 patients as circle and COVID-19 recovered as rhombus. Representative dot-plots showing Vδ1+ ( B ) and Vδ2+ ( D ) T lymphocyte memory subset distribution in hospitalized, recovered, and healthy subjects. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques: Ex Vivo, Expressing

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Analysis of exhaustion-marker expression by circulating Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and healthy subjects. Expression of TIM-3 and PD-1 on Vδ1+ ( A ) and Vδ2+ ( B ) T lymphocytes in hospitalized and recovered COVID-19 patients, compared to healthy donors. Shown are percentage ( left panels ) and MFI ( right panels ) ± SD. ( C ) Representative dot-plots of exhaustion-marker expression by Vδ1+ and Vδ2+ T lymphocytes from hospitalized and recovered COVID-19 patients, compared to healthy donors. ( D ) Frequency of exhausted Vδ1+ and Vδ2+ T cells per 1 × 10 6 T lymphocytes. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques: Marker, Expressing

Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Expression of pro-inflammatory cytokines by Vδ1+ T lymphocytes in hospitalized and recovered COVID-19 patients, compared to healthy subjects. Cumulative histograms representing the expression of pro-inflammatory cytokines by Vδ1+ T lymphocyte. Shown are percentage ( left panels ), MFI ( central panel , log 10 scale), and iMFI ( right panels ) ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: After the incubation, the cells were harvested, washed with FACS buffer and surface stained using the following fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), FITC-conjugated anti-human Vδ1 (clone REA173), or Vδ2 (clone REA771) (Miltenyi Biotec).

Techniques: Expressing

Journal: Cell Metabolism

Article Title: Regulation of Mother-to-Offspring Transmission of mtDNA Heteroplasmy

doi: 10.1016/j.cmet.2019.09.007

Figure Lengend Snippet:

Article Snippet: Mouse IgG (H+L) DyLight 800 , Rockland , 610-145-002.

Techniques: Recombinant, Modification, CyQUANT Assay, Proliferation Assay, Blocking Assay, Plasmid Preparation, Software

Journal: Toxins

Article Title: PTX Instructs the Development of Lung-Resident Memory T Cells in Bordetella pertussis Infected Mice

doi: 10.3390/toxins13090632

Figure Lengend Snippet: The generation of Trm cells is dependent on PTX exposure during B. pertussis infection. ( A ) Representative flow cytometry of CD69 and CD103 expression of CD62L − CD44 + CD4 + T cells harvested from lung in ΔPTX, pPTX, ΔPTX + pPTX and WT groups at day 15 p.i. Frequency and absolute numbers of ( B ) CD4 + Trm and ( C ) CD8 + Trm harvested in the lung of ΔPTX, pPTX, ΔPTX+pPTX and WT groups at day 15 p.i. Results are mean ± SD for at least 3 to 5 mice/group and are representative of two independent experiments. Statistical significance of differences are shown. * p -value < 0.05; ** p -value < 0.01, *** p -value < 0.001 and **** p -value < 0.0001.

Article Snippet: T cells were enriched negatively by using Pan T kit supplemented with CD62L antibody (MiltenyiBiotec, Paris, France).

Techniques: Infection, Flow Cytometry, Expressing